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Image Search Results
Journal: Cell Death & Disease
Article Title: Failure to resolve inflammation contributes to juvenile onset cardiac damage in a mouse model of Duchenne muscular dystrophy
doi: 10.1038/s41419-025-07816-5
Figure Lengend Snippet: A qRT-PCR analysis of a distinct cohort of D2- mdx and B10- mdx hearts to assess the expression of inflammatory genes identified by RNAseq analysis cohort to be differentially expressed. Transcripts include top dysregulated genes involved in leukocyte activation, migration and chemotaxis and regulation of inflammatory response and leukocyte-mediated immunity ( Ccl3, Ccl8, Stab2, Adam8, Trem2, Il7r ). Relative gene expression values normalized to internal Hprt transcript levels. B qRT-PCR analysis of a distinct cohort of D2- mdx and B10- mdx hearts to assess inflammatory genes that show broad dysregulation of neutrophil and macrophage response in juvenile D2- mdx hearts ( Il1b, Lgals3, Arg1, Fpr1, Fpr2, Anxa1 ). Relative gene expression values normalized to internal Hprt transcript levels. C , D Images showing immunostaining for pan-macrophage marker, F4/80 (green), and pro-inflammatory, pathogenic macrophage marker, GAL-3 (red), in juvenile D2- mdx ( C ) and B10- mdx ( D ). Data represent median ± IQR from n = 7−9 hearts per cohort. Statistical analyses performed using non-parametric Mann–Whitney test; ** p < 0.01, *** p < 0.001. For age-matched WT controls, refer to Supplementary Fig. .
Article Snippet: Specific mRNA transcript levels were quantified using individual TaqMan assays (
Techniques: Quantitative RT-PCR, Expressing, Activation Assay, Migration, Chemotaxis Assay, Gene Expression, Immunostaining, Marker, MANN-WHITNEY
Journal: BMC Immunology
Article Title: Phenotypic and functional stability of leukocytes from human peripheral blood samples: considerations for the design of immunological studies
doi: 10.1186/s12865-019-0286-z
Figure Lengend Snippet: Chemokine and cytokine gene expression in PBMCs. Gene expression of CXCL3 , CCL2 , CCR7 , IL-1ß , TNFα , CCL8 , CCR2 and CXCL10 ( a - h ), was measured by qRT-PCR. Data are presented as mean and statistical significance estimated by one-way ANOVA followed by Dunnett’s multiple comparison test. *: p < 0.05, **: p < 0.01, ***: p < 0.001
Article Snippet: Gene expression of inflammatory mediators was evaluated using Taqman probes (
Techniques: Gene Expression, Quantitative RT-PCR, Comparison